Figure 1: GFP-fragment reassembly vectors and cloning linker sequences. To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives ...

Covalent modification of the oncogene product Mdm2 by the ubiquitin-related protein SUMO1 protects it from ubiquitination and enhances its E3 ligase activity towards p53 in vitro. Disappearance of ...