Flow cytometry is an essential tool enabling precise cellular analysis across many fields, including immunology, oncology and cell biology research. However, suboptimal staining protocols can leave ...

Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data.

ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis of extracellular vesicles (EVs), where removing excess antibodies ...

Keep the staining reactions at as low a volume as possible to conserve reagents. For instance, you can add 25ml of a 2´ stock of stain to 25 ml of a 2´ stock of cells for a total volume of 50ml. All ...

A new system for automated flow cytometry sample preparation, the Laminar Wash™ AUTO 1000, offers scientists a fully-automated flow cytometry staining platform designed to produce the most ...

High-throughput flow cytometry of adherent cells is difficult because the creation of single cell suspensions can damage cells and yield artificial results. We describe a protocol to increase the ...

The simplest samples to prepare for flow cytometry are suspended culture cells, waterborne microorganisms, bacteria and yeast. Whole blood is also relatively easy to use, because the red blood cells ...

ABSTRACT: Protozoa play important roles in grazing and nutrient recycling, but quantifying these roles has been hindered by difficulties in collecting, culturing, and observing these often-delicate ...