Click image to enlarge. 4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 ...

Like PCR, qPCR amplifies DNA using a DNA polymerase enzyme.This adds new nucleotides to the 3’-OH end of a short, single-stranded DNA segment, called a primer, which complements the template strand. 2 ...

A direct amplification method, that simplifies and accelerates time to qPCR results, is a good solution to bypass the particularly rate-limiting step of RNA extraction. As the name implies, direct PCR ...

At the basic research-level, scientists rely on qPCR to evaluate changes in gene expression in varying conditions. qPCR success depends on careful primer design, sample and reagent purity, template ...

IDT's PrimeTime™ 1-Step 4X Broad-Range qPCR Master Mix is a unique, proprietary blend ideal for viral research and other gene expression applications.

It can generate 9,216 qPCR results in 4 hours. Learning Objectives. Understand how microfluidics enables high-throughput qPCR with singleplex accuracy. Understand the impact of miniaturization on ...