Additionally, GAS2 bundles microtubules and F-actin at low concentrations, suggesting a role in maintaining cell stiffness. Our findings reveal that the C-terminal region of GAS2 is essential for its ...

Additionally, we investigated the biophysical aspects of supplement therapy using Rho-associated protein kinase (ROCK) inhibitor (Y-27632) and myosin II inhibitor ((−)-blebbistatin), focusing on the ...

To explore potential cytoskeletal or cell shape regulation of RELA in PDAC, we constructed Bayesian models using single-cell datasets from TNFα-stimulated PDAC cells with variation in RELA, actin, and ...

Here, we show that a so far unexplored FAM110A protein has unique properties that allow it to bind actin and tubulin with the opposite ends and thus crosslink both cytoskeletal systems. FAM110A ...

We reported recently that β-tubulin isotype TUBB6 is key for cytoskeleton organization in osteoclasts and for bone resorption. Here, using an osteoclast model CRISPR/Cas9 KO for Tubb6, we show that ...

Currently, housekeeping proteins (HKPs) like actin, tubulin, or GAPDH are often used to check for equal loading or to compensate potential loading differences. However, these loading controls have ...

The tubulin family of proteins are vital components ... form a dimer that the microtubules are constructed from. Along with actin, microtubules are an essential component of the cytoskeleton ...

IIA1.6 B lymphoma cells were stimulated with BCR‐ligand − (anti‐IgM) or BCR‐ligand + (anti‐IgG) beads for 60 min, fixed and stained for F‐actin (top) and α‐tubulin (bottom). Scale bar: 3 μm. The graph ...

Vav/Cdc42 regulates actin polymerization and lysosomal synapse formation that ... media were purchased from Thermo Fisher Scientific (Waltham, MA). ZCL 278 and anti-tubulin antibody (clone DM1A) were ...

Abstract: We present the architecture, training strategy and evaluation of a convolutional neural network (CNN) designed for the segmentation of actin-stained cells in ... genetically distinct ...