This resource is designed for researchers, scientists, and drug development professionals to troubleshoot and minimize aspartimide formation, a critical side reaction in Fmoc-based SPPS.

Formation of a five-membered ring aspartimide through the attack of a backbone amide to the side chain of aspartate and asparagine residues is a long-known side-reaction in solid phase peptide synthesis, …

We demonstrate that it completely suppresses the formation of aspartimide and enhances ef ciency during SPPS. This approach improves the synthesis of a fi highly aspartimide-prone peptide...

In this study, different protecting groups and a variety of conditions using model peptide I were investigated systematically. To address the problem of incomplete Fmoc removal, harsher conditions …

Aug 26, 2025 · Given that optimized Boc-chemistry SPPS can already be used to synthesize peptides without aspartimide formation, it suggests an alternative approach to eliminate this side reaction, …

Primary Research Question: Can the thioimidate serve as a protecting group to prevent aspartimide formation? Confirmed via UPLC and NMR

This systematic investigation clearly showed that in our test system, no detectable amounts of aspartimide were formed if Hmb-backbone protection was applied in addition to standard OtBu …