Cyclin B1-Cdk1 complexes are maintained in an inactive state by association with p21, which provokes nuclear retention of cyclin B1-Cdk1 and prevents their activation at the centrosome …

In the presence of p53, p21 is induced and inhibits Cdk1, thereby contributing to prolong the G2/M arrest (Bunz et al., 1998). Activation of the DNA damage checkpoint and a transient cell cycle …

Non-phosphorylatable p21 binds strongly to Cdk1/cyclin B1. To gain insight into the binding affinity of p21 and its mutants to Cdk1/cyclin B1, pulldown assays were performed using …

This nuclear retention, which strictly depends on association with endogenous p21, prevents activation of cyclin B1-Cdk1 by Cdc25 and Cdk-activating kinase as well as its recruitment to …

Aug 2, 2016 · The multifunctional protein p21Cip1/CDKN1A (p21) is an important and universal Cdk-interacting protein. Recently, we have reported that p21 is involved in the regulation of the …

Loss of cell cycle control is characteristic of tumorigenesis. The protein p21 is the founding member of cyclin-dependent kinase inhibitors and an important versatile cell cycle protein. p21 …

Here we present evidence that this is possible due to premature translocation of Cdk1 from the cytoplasm to the nucleus in the absence of Cdk2. We also investigated the consequence of …

Jun 4, 2004 · The critical role of p21 in nuclear sequestration and blocking activation of cyclin B1-Cdk1 is underscored by our results showing that exposure of p21-deficient or null fibroblasts to …

Nov 9, 2020 · CDKN1A, encoding for the CDK inhibitor p21 CIP1, represents a critical target gene of p53/p73. Loss of p21 CIP1 unleashes CDK1 activity which causes W-CIN in otherwise …

Oct 17, 2007 · Our study demonstrates that p21 binds to Cdk1 in the absence of Cdk2 to arrest cell cycle progression in response to irradiation. Our immunofluorescence and …